Tripartite Symbiotic Digestion of Lignocellulose in the Digestive System of a Fungus-Growing Termite

Fungus-growing termites are efficient in degrading and digesting plant substrates, achieved through the engagement of symbiotic gut microbiota and lignocellulolytic Termitomyces fungi cultivated for protein-rich food. Insights into where specific plant biomass components are targeted during the decomposition process are sparse. In this study, we performed several analytical approaches on the fate of plant biomass components and did amplicon sequencing of the 16S rRNA gene to investigate the lignocellulose digestion in the symbiotic system of the fungus-growing termite Odontotermes formosanus (Shiraki) and to compare bacterial communities across the different stages in the degradation process. We observed a gradual reduction of lignocellulose components throughout the process. Our findings support that the digestive tract of young workers initiates the degradation of lignocellulose but leaves most of the lignin, hemicellulose, and cellulose, which enters the fresh fungus comb, where decomposition primarily occurs. We found a high diversity and quantity of monomeric sugars in older parts of the fungus comb, indicating that the decomposition of lignocellulose enriches the old comb with sugars that can be utilized by Termitomyces and termite workers. Amplicon sequencing of the 16S rRNA gene showed clear differences in community composition associated with the different stages of plant biomass decomposition which could work synergistically with Termitomyces to shape the digestion process.

Automated summary

This study investigated the digestion of lignocellulose in the symbiotic system of fungus-growing termites. The results showed that young workers initiate the degradation of lignocellulose, while the majority of lignin, hemicellulose, and cellulose enter the fresh fungus comb for decomposition. The decomposition of lignocellulose enriches the old comb with sugars that can be utilized by Termitomyces and termite workers. Additionally, clear differences in bacterial community composition were observed at different stages of plant biomass decomposition, which may interact synergistically with Termitomyces to shape the digestion process.