4.7 Article

Propofol and α2-Agonists Attenuate Microglia Activation and Restore Mitochondrial Function in an In Vitro Model of Microglia Hypoxia/Reoxygenation

Journal

ANTIOXIDANTS
Volume 11, Issue 9, Pages -

Publisher

MDPI
DOI: 10.3390/antiox11091682

Keywords

microglia; inflammation: mitochondria; hypoxia; propofol and alpha 2-agonists

Funding

  1. Piano di Incentivi per la ricercar di Ateneo
  2. PON AIM RI [2014-2020-E66C18001240007]

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This study evaluated the effects of Dexmedetomidine, Clonidine, and Propofol in a model of hypoxia/reoxygenation injury. The results showed that these drugs can attenuate oxidative stress and inflammation, and restore energy metabolism, with different neuroprotective profiles.
Cerebrovascular ischemia is a common clinical disease encompassing a series of complex pathophysiological processes in which oxidative stress plays a major role. The present study aimed to evaluate the effects of Dexmedetomidine, Clonidine, and Propofol in a model of hypoxia/reoxygenation injury. Microglial cells were exposed to 1%hypoxia for 3 h and reoxygenated for 3 h, and oxidative stress was measured by ROS formation and the expression of inflammatory process genes. Mitochondrial dysfunction was assessed by membrane potential maintenance and the levels of various metabolites involved in energetic metabolism. The results showed that Propofol and alpha 2-agonists attenuate the formation of ROS during hypoxia and after reoxygenation. Furthermore, the alpha 2-agonists treatment restored membrane potential to values comparable to the normoxic control and were both more effective than Propofol. At the same time, Propofol, but not alpha 2-agonists, reduces proliferation (Untreated Hypoxia = 1.16 +/- 0.2, Untreated 3 h Reoxygenation = 1.28 +/- 0.01 vs. Propofol hypoxia = 1.01 +/- 0.01 vs. Propofol 3 h Reoxygenation = 1.12 +/- 0.03) and microglial migration. Interestingly, all of the treatments reduced inflammatory gene and protein expressions and restored energy metabolism following hypoxia/reoxygenation (ATP content in hypoxia/reoxygenation 3 h: Untreated = 3.11 +/- 0.8 vs. Propofol = 7.03 +/- 0.4 vs. Dexmedetomidine = 5.44 +/- 0.8 vs. Clonidine = 7.70 +/- 0.1), showing that the drugs resulted in a different neuroprotective profile. In conclusion, our results may provide clinically relevant insights for neuroprotective strategies in intensive care units.

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