Freeze-drying platforms design for batch fabrication of Haversian system mimicking scaffolds with enhanced osteogenesis

The Haversian system is one of the most important pathways to repair bone defects, and it is the basic guarantee for the repair of bone defects, which means that the formation of the Haversian system indicates repairing of the defects. The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, in contrast to the increasing demands, how to rapidly prepare various sizes of such Haversian system mimicking scaffolds in batch becomes a major challenge. In this study, we designed three types of platforms with different sizes in combination with the freeze-drying approach. Chitosan/type I collagen composite materials were used to study the structure, morphology, and performance of the production, and the effects of the controlled architecture on osteogenesis. Results showed that the physicochemical effects of the mass fabricated scaffolds of various sizes met the requirements of bone repair materials. In addition, the scaffolds had good cytocompatibility and excellent in vivo bone repair performance, which have potential clinical applications.

Automated summary

The Haversian system plays a crucial role in bone defect repair and serves as the fundamental guarantee for the repair process. This study focused on the development of tissue engineering scaffolds that mimic the Haversian system, emphasizing the integration of structure and function. By utilizing a freeze-drying approach, three types of platforms with different sizes were designed. The results revealed that the mass fabricated scaffolds met the requirements for bone repair materials and exhibited excellent cytocompatibility and in vivo bone repair performance, suggesting their potential clinical applications.