Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.957268
Keywords
microalgae; biorefinery; protein; membrane ultrafiltration; depth filtration
Funding
- Texas A&M Agrilife Research
- Qualitas Health Inc
- [M1802950]
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This study evaluates tangential flow ultrafiltration as an alternative method for producing protein hydrolysates. The results show that the membrane diafiltration process has the potential to efficiently separate and recover hydrolyzed protein, with a higher protein recovery yield compared to centrifugation and depth filtration methods. Additionally, protein hydrolysates obtained by membrane ultrafiltration exhibit slightly better heat and pH stability.
Protein hydrolysates are one of the most valuable products that can be obtained from lipid-extracted microalgae (LEA). The advantages of protein hydrolysates over other protein products encompass enhanced solubility, digestibility, and potential bioactivity. The development of an economically feasible process to produce protein hydrolysates depends on maximizing the recovery of hydrolyzed native protein from the lipid-extracted algal biomass and subsequent fractionation of hydrolyzed protein slurry. Previously, we reported a method for fractionation of enzymatically generated protein hydrolysates by acidic precipitation of algal cell debris and unhydrolyzed protein, precipitate wash, centrifugation, and depth filtration. The present study evaluates tangential flow ultrafiltration as a single-step alternative to centrifugation, precipitate wash, and depth filtration. The results demonstrate that the tangential flow ultrafiltration process has a potential that deserves further investigation. First, the membrane diafiltration process uses a single and easily scalable unit operation (tangential flow filtration) to separate and wash out hydrolyzed protein from the algal residue. Second, the protein recovery yield achieved with the tangential flow process was > 70% compared to 64% previously achieved by centrifugation and depth filtration methods. Finally, protein hydrolysates obtained by membrane ultrafiltration exhibited slightly better heat and pH stability.
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