Effects of Shrub Encroachment in the Inner Mongolia Ecotones between Forest and Grassland on the Species Diversity and Interspecific Associations

Shrub encroachment, which is the increase in shrubs or woody plants in grassland, is one of the important ecological problems facing grassland worldwide. The study was conducted in the ecotones between the forest and grassland of Inner Mongolia. Using a simple random sampling method, 40 shrubland sample plots, evenly distributed in the typical forest-grass transition area, were selected for community investigation. According to the steppe shrub encroachment index, the grassland was divided into different levels. The species diversity and interspecific association of different levels of sample plots were statistically analyzed and compared. It was found that the Shannon-Wiener index (H) and Simpson index (P) were negatively associated with the shrub encroachment index (p < 0.01) and gradually decreased with increasing levels of shrub encroachment. When the grassland transitioned to severe shrub formation, the species diversity of the community significantly decreased, and H and P were significantly lower than those of the mild and moderate shrub-steppe, and the lowest values were 1.37 and 0.48, respectively. With the increase in the shrub encroachment index, biomass showed a trend of first increasing and then decreasing. The aboveground biomass of shrub interspace and total aboveground biomass in the severe shrub encroachment steppe were significantly lower than those in the mildly and moderately shrub-steppe, with the lowest values of 101.86 g/m(2) and 189.69 g/m(2), respectively. Shrub encroachment led to a change in the overall association of shrub communities in the ecotones between the forest and grassland of Inner Mongolia from positive to negative. The vast majority of species pairs in the shrub community showed no significant association, and the interspecies association was relatively weak. The results showed that shrub encroachment would affect community species diversity; mild and moderate shrub encroachment had no significant impact on community species diversity, but severe shrub encroachment significantly reduced the community species diversity. There was no significant effect of shrub encroachment on aboveground biomass, which increased first and then decreased, and the herbaceous plant population played a leading role in grassland productivity. The interspecific association of grassland is loose and weak. The community was in the early stage of unstable succession, and it was possible to continue shrub encroachment or reverse succession into a typical grassland in response to the interference of human or environmental factors.

Automated summary

This study conducted in the ecotones between the forest and grassland of Inner Mongolia found that shrub encroachment has an impact on species diversity in grassland, with severe shrub formation significantly reducing species diversity. However, shrub encroachment does not significantly affect aboveground biomass, with herbaceous plant population playing a leading role in grassland productivity. Additionally, shrub encroachment leads to a change in the overall association of shrub communities from positive to negative.