Journal
OPEN FORUM INFECTIOUS DISEASES
Volume 9, Issue 10, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ofid/ofac490
Keywords
COVID-19; digital PCR; international units; SARS-CoV-2; viral load
Categories
Funding
- American Lebanese Syrian Associated Charities
- St. Jude Children's Research Hospital
- National Institute of Allergy and Infectious Diseases (NIAID) [HHSN27220140006C]
- NIAID Collaborative Influenza Vaccine Innovation Centers [75N93019C00052, 3U01AI144616-02S1]
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This study validates a quantitative SARS-CoV-2 digital PCR assay and correlates viral RNA load with symptoms and disease severity. The standardized method of measuring viral load in SARS-CoV-2 infection provided by this study is significant.
Studies have evaluated SARS-CoV-2 infection using Cycle Thresholds (Ct) values as a surrogate of viral RNA load. We validated a quantitative SARS-CoV-2 digital PCR assay normalized to WHO International Units and correlated viral RNA load with symptoms and disease severity. Although numerous studies have evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using cycle threshold (Ct) values as a surrogate of viral ribonucleic acid (RNA) load, few studies have used standardized, quantitative methods. We validated a quantitative SARS-CoV-2 digital polymerase chain reaction assay normalized to World Health Organization International Units and correlated viral RNA load with symptoms and disease severity.
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