Journal
PHARMACEUTICS
Volume 14, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics14102060
Keywords
Aloe vera; broth microdilution; fibroblasts; phenomenological combination index; Porphyromonas gingivalis; promigratoy effect; Rheum palmatum root extract; scratch assay; synergy; wound healing
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Funding
- BiomaTiCS research initiative of the University Medical Center, Mainz
- ministry of economics Rhineland-Palatinate
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This study investigated the effect of Aloe vera and Rheum palmatum root on oral wound healing. The results showed that they can promote fibroblast migration and have antimicrobial efficacy against Porphyromonas gingivalis. These compounds could be potential topical treatments for wound healing disorders.
Various local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of Aloe vera (AV) and Rheum palmatum root (RPR) on oral wound healing capacity in vitro. The effect of AV and RPR on human primary fibroblast viability and migration was studied by measuring metabolic activity and gap closure in a scratch assay. Furthermore, cell cycle distribution and cytoskeletal features were analyzed. Antimicrobial activity against the oral pathogen Porphyromonas gingivalis was evaluated by broth microdilution assay. AV and RPR increased fibroblast migration after single agent treatment. Synergistic effects of the plant extract combination were observed regarding cellular migration which were confirmed by calculation of the phenomenological combination index (pCI), whereas the cell cycle distribution was not influenced. Furthermore, the combination of AV and RPR showed synergistic antibacterial effects as determined by the fractional inhibitory concentration index. This study demonstrated that the combination of AV and RPR can promote the migration of human primary fibroblasts in vitro and exert antimicrobial efficacy against P. gingivalis, suggesting these compounds for the topical treatment of wound healing disorders.
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