Journal
CELLS
Volume 11, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/cells11182841
Keywords
basic fibroblast growth factor; epidermal growth factor; vitamin A; enteric neural stem cell; gangliogenesis; neural network; neurosphere
Categories
Funding
- Chang Gung Medical Foundation [CMRPG3K0432, CMRPG3J1112, CMRPG3M0191]
Ask authors/readers for more resources
This study explores the effects of neurosphere medium (NSM) and self-renewal medium (SRM) on the development of enteric neural stem cells (ENSCs) in vitro. The results show that the different compositions of NSM and SRM lead to distinct outcomes, with epidermal growth factor inhibiting enteric neuronal wiring in NSM, while basic fibroblast growth factor promotes neurogenesis and gangliogenesis in ENSCs.
Extending well beyond the original use of propagating neural precursors from the central nervous system and dorsal root ganglia, neurosphere medium (NSM) and self-renewal medium (SRM) are two distinct formulas with widespread popularity in enteric neural stem cell (ENSC) applications. However, it remains unknown what growth factors or nutrients are crucial to ENSC development, let alone whether the discrepancy in their components may affect the outcomes of ENSC culture. Dispersed enterocytes from murine fetal gut were nurtured in NSM, SRM or their modifications by selective component elimination or addition to assess their effects on ENSC development. NSM generated neuriteless neurospheres, whereas SRM, even deprived of chicken embryo extract, might wire ganglia together to assemble neural networks. The distinct outcomes came from epidermal growth factor, which inhibited enteric neuronal wiring in NSM. In contrast, basic fibroblast growth factor promoted enteric neurogenesis, gangliogenesis, and neuronal wiring. Moreover, vitamin A derivatives might facilitate neuronal maturation evidenced by p75 downregulation during ENSC differentiation toward enteric neurons to promote gangliogenesis and network assembly. Our results might help to better manipulate ENSC propagation and differentiation in vitro, and open a new avenue for the study of enteric neuronal neuritogenesis and synaptogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available