Journal
CELLS
Volume 11, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/cells11193065
Keywords
hyaluronic acid; milk exosomes; tumor-specific delivery; CD44; microRNA
Categories
Funding
- Social Development Key Project of Jiangsu Province [BE2019632]
- Natural Science Foundation of Jiangsu Province [BK20200601]
- National Natural Science Foundation of China [21907038, 32000904]
- Wuxi Taihu Lake Talent Plan
- Key Medical Discipline of Wuxi [ZDXK2021002]
- Health and Family Planning Commission ofWuxi, China [Z202005, Q202017]
- 111 Project [111-2-06]
Ask authors/readers for more resources
Cell type-specific drug delivery using hyaluronic acid (HA) coated bovine milk exosomes (HA-mExo) shows promising results for targeted cancer therapy. The HA coating allows for specific targeting of CD44-positive cancer cells, leading to increased uptake of microRNA-204-5p mimics (miR-204). Compared to uncoated exosomes, HA-mExo demonstrates significantly enhanced antitumor efficacy with excellent biocompatibility.
Cell type-specific drug delivery is a straightforward strategy to achieve targeted cancer therapy and reduce side effects. Hyaluronic acid (HA), an U.S. Food and Drug Administration (FDA)-approved biocompatible carbohydrate polymer, has been extensively employed as a targeting ligand for a drug delivery system due to its natural ability to bind to tumor cells overexpressing cluster of differentiation 44 (CD44) receptors. Here, we report the preparation and antitumor efficacy of HA-coated bovine milk exosomes (HA-mExo) for tumor-specific delivery of microRNA-204-5p mimics (miR-204). The exosome-based delivery formulation was prepared with miR-204 encapsulated inside the lumen and HA displayed outside the membrane. The resultant formulation of HA-mExo-miR204 was able to specifically target CD44-positive cancer cells, with a concomitant increase in the intracellular uptake of miR-204. Compared to the uncoated mExo-miR204 formulation, HA-mExo-miR204 showed significantly increased antitumor efficacy both in vitro and in vivo. Importantly, HA-mExo-miR204 showed excellent biocompatibility and did not cause significant systemic toxicity. Given that both HA and bovine milk exosomes are low-cost and highly accessible biogenic materials with broad biomedical applications, HA-decorated bovine milk exosomes can be proven to be a practical drug delivery system of RNA drugs for targeted cancer therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available