Journal
CELLS
Volume 11, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/cells11182836
Keywords
cell recognition; fluorescent probes; high-throughput screening; mitochondria
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Funding
- Institute for Basic Science (IBS) [IBS-R007-A1]
- POSTECH (Pohang University of Science and Technology)
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In this study, a novel human-T-cell-selective fluorescent probe was developed, which effectively distinguished T cells from B cells based on the differences in mitochondrial mass and membrane potential. This study enriches the methods for live cell distinction from complex cell communities.
The identification of T and B lymphocytes has relied on using antibodies against different biomarkers as the gold standard. Emerging small molecule-based fluorescent probes have the potential to replace antibodies. Herein, we report the first human-T-cell-selective fluorescent probe, Mito thermo yellow (MTY), achieving the live T cells' distinction from B cells, which was previously impossible without the help of antibodies. The unexpected cell selectivity of MTY is attributed to the higher mitochondria mass and membrane potential of T cells over B cells. This study enriches the toolbox for live cell distinction from complex cell communities.
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