4.8 Article

Intermediate cells of in vitro cellular reprogramming and in vivo tissue regeneration require desmoplakin

Journal

SCIENCE ADVANCES
Volume 8, Issue 43, Pages -

Publisher

AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abk1239

Keywords

-

Funding

  1. National Research Foundation of Korea [2019R1A2C2087606, 2018R1A5A1025511]
  2. Samsung Research Funding Center of Samsung Electronics [SRFC-MA1601-06]
  3. National Research Council of Science and Technology (NST) of Ministry of Science and ICT of Korea [CRC-15-04-KIST]
  4. KBRI basic research program through Korea Brain Research Institute [22-BR-01-03]
  5. KRIBB research initiative program [KGM4722223, KGM5362212, KGM5192113, KGM9992211, KGM5352113, KGM2112133]
  6. National Research Foundation of Korea [2019R1A2C2087606] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

Ask authors/readers for more resources

The cytoskeletal linker protein desmoplakin (Dsp) plays a crucial role in both in vitro cellular reprogramming and tissue regeneration. Knockdown of Dsp impairs the formation of intermediate cells during cellular reprogramming and tissue regeneration. This study suggests a potential evolutionary link mediated by Dsp between cellular reprogramming in mammals and tissue regeneration in lower vertebrates, and the intermediate cells may provide alternative approaches for mammalian regenerative therapy.
Amphibians and fish show considerable regeneration potential via dedifferentiation of somatic cells into blastemal cells. In terms of dedifferentiation, in vitro cellular reprogramming has been proposed to share common processes with in vivo tissue regeneration, although the details are elusive. Here, we identified the cytoskeletal linker protein desmoplakin (Dsp) as a common factor mediating both reprogramming and regeneration. Our analysis revealed that Dsp expression is elevated in distinct intermediate cells during in vitro reprogramming. Knockdown of Dsp impedes in vitro reprogramming into induced pluripotent stem cells and induced neural stem/progenitor cells as well as in vivo regeneration of zebrafish fins. Notably, reduced Dsp expression impairs formation of the intermediate cells during cellular reprogramming and tissue regeneration. These findings suggest that there is a Dsp-mediated evolutionary link between cellular reprogramming in mammals and tissue regeneration in lower vertebrates and that the intermediate cells may provide alternative approaches for mammalian regenerative therapy.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available