Journal
SCIENCE ADVANCES
Volume 8, Issue 35, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abn6027
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Funding
- Office of the Vice Chancellor for Research at Washington University in St. Louis
- Diabetes Research Center [NIH P30DK020579]
- NIH [R01DK058366, R01DK078314, R01DK128660, R01DK131177]
- National Science Foundation Center for Engineering Mechanobiology [CMMI 1548571]
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Chronic kidney diseases are widespread and incurable, and the underlying biophysical mechanisms are unclear. Sarcomere-like structures (SLSs) have been identified in injured kidney podocytes, and they may play a role in the regeneration process. Results from an ex vivo culture system supported this hypothesis, suggesting SLSs as potential mechanobiological mediators for podocyte recovery.
Chronic kidney diseases are widespread and incurable. The biophysical mechanisms underlying them are unclear, in part because material systems for reconstituting the microenvironment of relevant kidney cells are limited. A critical question is how kidney podocytes (glomerular epithelial cells) regenerate foot processes of the filtration apparatus following injury. Recently identified sarcomere-like structures (SLSs) with periodically spaced myosin IIA and synaptopodin appear in injured podocytes in vivo. We hypothesized that SLSs template synaptopodin in the initial stages of recovery in response to microenvironmental stimuli and tested this hypothesis by developing an ex vivo culture system that allows control of the podocyte microenvironment. Results supported our hypothesis. SLSs in podocytes that migrated from isolated kidney glomeruli presented periodic synaptopodin-positive clusters that nucleated peripheral, foot process-like extensions. SLSs were mechanoresponsive to actomyosin inhibitors and substrate stiffness. Results suggest SLSs as mechanobiological mediators of podocyte recovery and as potential targets for therapeutic intervention.
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