4.4 Article

Mobilisation of HLA-F on the surface of bronchial epithelial cells and platelets in asthmatic patients

Journal

HLA
Volume 100, Issue 5, Pages 491-499

Publisher

WILEY
DOI: 10.1111/tan.14782

Keywords

asthma; bronchial epithelial cells; cell activation; HLA-F; mobilisation; platelets

Funding

  1. Fondation du Souffle, Fonds de Dotation Recherche en Sante Respiratoire
  2. Fondation du Souffle

Ask authors/readers for more resources

Uncontrolled inflammation in chronic obstructive lung diseases, such as asthma, results in respiratory exacerbation and dysfunction. This study investigates the expression of HLA-F, a molecule involved in immune cell activation, in human bronchial epithelium cells (HBEC) and platelets (PLT). The results suggest that HLA-F is expressed in both cell types under healthy conditions and is upregulated in the context of asthma-associated inflammation. These findings highlight the potential therapeutic relevance of HLA-F in controlling lung inflammation.
Uncontrolled inflammation of the airways in chronic obstructive lung diseases leads to exacerbation, accelerated lung dysfunction and respiratory insufficiency. Among these diseases, asthma affects 358 million people worldwide. Human bronchial epithelium cells (HBEC) express both anti-inflammatory and activating molecules, and their deregulated expression contribute to immune cell recruitment and activation, especially platelets (PLT) particularly involved in lung tissue inflammation in asthma context. Previous results supported that HLA-G dysregulation in lung tissue is associated with immune cell activation. We investigated here HLA-F expression, reported to be mobilised on immune cell surface upon activation and displaying its highest affinity for the KIR3DS1-activating NK receptor. We explored HLA-F transcriptional expression in HBEC; HLA-F total expression in PBMC and HBEC collected from healthy individuals at rest and upon chemical activation and HLA-F membrane expression in PBMC, HBEC and PLT collected from healthy individuals at rest and upon chemical activation. We compared HLA-F transcriptional expression in HBEC from healthy individuals and asthmatic patients and its surface expression in HBEC and PLT from healthy individuals and asthmatic patients. Our results support that HLA-F is expressed by HBEC and PLT under healthy physiological conditions and is retained in cytoplasm, barely expressed on the surface, as previously reported in immune cells. In both cell types, HLA-F reaches the surface in the inflammatory asthma context whereas no effect is observed at the transcriptional level. Our study suggests that HLA-F surface expression is a ubiquitous post-transcriptional process in activated cells. It may be of therapeutic interest in controlling lung inflammation.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available