Bioprocessing of pineapple waste biomass for sustainable production of bioactive compounds with high antioxidant activity

The effect of temperature, moisture content and pH during solid-state fermentation (SSF) of MD2 pineapple peel with Rhizopus oryzae (MUCL 28168) was evaluated on the release of bioactive compounds with antioxidant capacity. Applying a central composite design, it was found that temperature had a significant effect (p < 0.05) on the total phenolic content and DPPH antioxidant activity while for the ABTS radical elimination activity, the factor that presented a significant effect was the pH (p < 0.05); as this factor increases, the antioxidant activity enhances. The optimal conditions for fermentation process were 80% of moisture content, pH 5.5, temperature 37.3 degrees C and 24 h of process to maximize phenolic content and antioxidant activity. Gallic acid, chlorogenic acid, caffeic acid and cinnamic acid were identified in the extracts by HPLC analysis. These results permit to conclude that SSF of pineapple peel is an effective bioprocess for the release of phenolic compounds with antioxidant activity. [GRAPHICS] .

Automated summary

The effects of temperature, moisture content, and pH on the release of bioactive compounds with antioxidant capacity during solid-state fermentation of MD2 pineapple peel with Rhizopus oryzae (MUCL 28168) were evaluated. The results showed that temperature had a significant effect on total phenolic content and DPPH antioxidant activity, while pH had a significant effect on ABTS radical elimination activity.