A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening

Homeodomain-leucine zipper (HD-Zip) transcription factors are only present in higher plants and are involved in plant development and stress responses. However, our understanding of their participation in the fruit ripening of economical plants, such as tomato (Solanum lycopersicum), remains largely unclear. Here, we report that VAHOX1, a member of the tomato HD-Zip I subfamily, was expressed in all tissues, was highly expressed in breaker+4 fruits, and could be induced by ethylene. RNAi repression of VAHOX1 (VAHOX1-RNAi) resulted in accelerated fruit ripening, enhanced sensitivity to ethylene, and increased total carotenoid content and ethylene production. Conversely, VAHOX1 overexpression (VAHOX1-OE) in tomato had the opposite effect. RNA-Seq results showed that altering VAHOX1 expression affected the transcript accumulation of a series of genes involved in ethylene biosynthesis and signal transduction and cell wall modification. Additionally, a dual-luciferase reporter assay, histochemical analysis of GUS activity and a yeast one-hybrid (Y1H) assay revealed that VAHOX1 could activate the expression of AP2a. Our findings may expand our knowledge about the physiological functions of HD-Zip transcription factors in tomato and highlight the diversities of transcriptional regulation during the fruit ripening process.

Automated summary

In this study, the tomato HD-Zip transcription factor VAHOX1 was found to be involved in fruit ripening. Suppression of VAHOX1 accelerated fruit ripening, increased sensitivity to ethylene, and enhanced carotenoid content and ethylene production in tomato fruits. Conversely, overexpression of VAHOX1 had the opposite effect. VAHOX1 affected the expression of genes involved in ethylene biosynthesis, signal transduction, and cell wall modification. The findings expand our knowledge of the physiological functions of HD-Zip transcription factors in tomato and highlight the diversity of transcriptional regulation during fruit ripening.