Journal
FRONTIERS IN IMMUNOLOGY
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.1005551
Keywords
chemokine receptors; Cxcr2; neutrophils; inflammation; tumor microenvironment
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The chemokine receptor Cxcr2 plays a crucial role in neutrophil physiology, as evidenced by the findings from Cxcr2 knockout mice. Cxcr2 deficiency leads to decreased percentage of mature neutrophils in the spleen and increased accumulation of aged neutrophils. It also affects their phagocytic ability and signaling pathways.
Neutrophils play a major role in the protection from infections but also in inflammation related to tumor microenvironment. However, cell-extrinsic and -intrinsic cues driving their function at steady state is still fragmentary. Using Cxcr2 knock-out mice, we have evaluated the function of the chemokine receptor Cxcr2 in neutrophil physiology. We show here that Cxcr2 deficiency decreases the percentage of mature neutrophils in the spleen, but not in the bone marrow (BM). There is also an increase of aged CD62L(lo) CXCR4(hi) neutrophils in the spleen of KO animals. Spleen Cxcr2(-/-) neutrophils display a reduced phagocytic ability, whereas BM neutrophils show an enhanced phagocytic ability compared to WT neutrophils. Spleen Cxcr2(-/-) neutrophils show reduced reactive oxygen species production, F-actin and alpha-tubulin levels. Moreover, spleen Cxcr2(-/-) neutrophils display an altered signaling with reduced phosphorylation of ERK1/2 and p38 MAPK, impaired PI3K-AKT, NF-kappa B, TGF beta and IFN gamma pathways. Altogether, these results suggest that Cxcr2 is essential for neutrophil physiology.
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