Metal Ion Doping of Alginate-Based Surface Coatings Induces Adipogenesis of Stem Cells

Metal ions are important effectors of protein and cell functions. Here, polyelectrolyte multilayers (PEMs) made of chitosan (Chi) and alginate (Alg) were doped with different metal ions (Ca2+, Co2+, Cu2+, and Fe3+), which can form bonds with their functional groups. Ca2+ and Fe3+ ions can be deposited in PEM at higher quantities resulting in more positive zeta potentials and also higher water contact angles in the case of Fe3+. An interesting finding was that the exposure of PEM to metal ions decreases the elastic modulus of PEM. Fourier transformed infrared (FTIR) spectroscopy of multilayers provides evidence of interaction of metal ions with the carboxylic groups of Alg but not for hydroxyl and amino groups. The observed changes in wetting and surface potential are partly related to the increased adhesion and proliferation of multipotent C3H10T1/2 fibroblasts in contrast to plain nonadhesive [Chi/Alg] multilayers. Specifically, PEMs doped with Cu2+ and Fe3+ ions greatly promote cell attachment and adipogenic differentiation, which indicates that changes in not only surface properties but also the bioactivity of metal ions play an important role. In conclusion, metal ion-doped multilayer coatings made of alginate and chitosan can promote the differentiation of multipotent cells on implants without the use of other morphogens like growth factors.

Automated summary

Metal ions can significantly affect protein and cell functions. In this study, polyelectrolyte multilayers (PEMs) made of chitosan and alginate were doped with different metal ions (Ca2+, Co2+, Cu2+, and Fe3+), resulting in changes in surface properties, such as zeta potentials and water contact angles. Metal ions, especially Cu2+ and Fe3+, had a positive effect on cell adhesion and differentiation, indicating the importance of bioactivity. These metal ion-doped coatings have the potential to promote the differentiation of multipotent cells on implants, without the need for additional growth factors.