Proteomic response of early juvenile Pacific oysters (Crassostrea gigas) to temperature

Pacific oysters (Crassostrea gigas) are a valuable aquaculture product that provides important ecosystem benefits. Among other threats, climate-driven changes in ocean temperature can impact oyster metabolism, survivorship, and immune function. We investigated how elevated temperature impacts larval oysters during settlement (19-33 days post-fertilization), using shotgun proteomics with data-independent acquisition to identify proteins present in the oysters after 2 weeks of exposure to 23 degrees C or 29 degrees C. Oysters maintained at elevated temperatures were larger and had a higher settlement rate, with 86% surviving to the end of the experiment; these oysters also had higher abundance trends of proteins related to metabolism and growth. Oysters held at 23 degrees C were smaller, had a decreased settlement rate, displayed 100% mortality, and had elevated abundance trends of proteins related to immune response. This novel use of proteomics was able to capture characteristic shifts in protein abundance that hint at important differences in the phenotypic response of Pacific oysters to temperature regimes. Additionally, this work has produced a robust proteomic product that will be the basis for future research on bivalve developmental processes.

Automated summary

Elevated temperature has significant impacts on Pacific oysters, affecting their metabolism, growth, and immune response. Oysters kept at higher temperatures showed faster growth, higher settlement rate, and increased survival with proteins related to metabolism; while those kept at lower temperatures exhibited smaller size, lower settlement rate, and higher abundance of proteins related to immune response. This study highlights the importance of proteomics in understanding the phenotypic response of oysters to temperature changes.