Low Toxicity of Metal-Organic Framework MOF-74(Co) Nano-Particles In Vitro and In Vivo

With the rapid development of metal-organic frameworks (MOF), the toxicity and environmental safety of MOF materials should be thoroughly investigated. The behaviors and bio-effects of MOF materials after oral exposure are largely unknown. In this study, we performed a pilot toxicity evaluation of MOF-74(Co) nanoparticles (NPs) both in vitro and in vivo. The cell viability and cell cycle were monitored after LO2 cells were incubated with MOF-74(Co). The Co contents, bodyweight, serum biochemistry, histopathological changes, and oxidative stress parameters were measured after oral exposure to MOF-74(Co) NPs in mice. LO2 cells showed viability loss at 100 mg/L. The cell cycle arrest was more sensitive, which was observed even at 12.5 mg/L. MOF-74(Co) NPs led to a significant accumulation of Co in the liver and kidneys. No bodyweight loss was observed and the serum biochemical index was mainly unchanged. Except for slight inflammation, the histopathological images of the liver and kidneys after oral exposure to MOF-74(Co) NPs were normal compared to the control. Meaningful oxidative stress was found in the liver and kidneys. The results collectively indicated the low toxicity of MOF-74(Co) NPs after oral exposure in mice.

Automated summary

The study found that oral exposure to MOF-74(Co) NPs can lead to significant accumulation of Co in the liver and kidneys, but overall has low toxicity to mice. Cell viability is lost at 100 mg/L, while cell cycle arrest can occur even at 12.5 mg/L. Apart from slight inflammation, the histopathological images of the liver and kidneys after oral exposure to MOF-74(Co) NPs were normal compared to the control group.