4.7 Article

Two-dimensional Ti3C2Tx MXene promotes electrophysiological maturation of neural circuits

Journal

JOURNAL OF NANOBIOTECHNOLOGY
Volume 20, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12951-022-01590-8

Keywords

Neural stem cells; Neuronal development and maturation; Ti3C2Tx MXene; Patch-clamp recording; Neural spiking; Synaptic transmission

Funding

  1. National Key R&D Program of China [2021YFA1101300, 2020YFA0112503]
  2. Strategic Priority Research Program of the Chinese Academy of Science [XDA16010303]
  3. National Natural Science Foundation of China [82030029, 81970882, 92149304, 82171141]
  4. Natural Science Foundation from Jiangsu Province [BE2019711]
  5. Science and Technology Department of Sichuan Province [2021YFS0371]
  6. Shenzhen Fundamental Research Program [JCYJ20190814093401920, JCYJ20210324125608022]
  7. Open Research Fund of State Key Laboratory of Genetic Engineering, Fudan University [SKLGE2104]
  8. Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX21_0080]

Ask authors/readers for more resources

In this study, the effects of Ti3C2Tx MXene on the morphological and electrophysiological properties of neural stem cell (NSC)-derived neurons were investigated. The results showed that Ti3C2Tx MXene promotes NSCs differentiation and neurite growth, enhances synaptic transmission between mature neurons.
Background The ideal neural interface or scaffold for stem cell therapy shall have good biocompatibility promoting survival, maturation and integration of neural stem cells (NSCs) in targeted brain regions. The unique electrical, hydrophilic and surface-modifiable properties of Ti3C2Tx MXene make it an attractive substrate, but little is known about how it interacts with NSCs during development and maturation. Results In this study, we cultured NSCs on Ti3C2Tx MXene and examined its effects on morphological and electrophysiological properties of NSC-derived neurons. With a combination of immunostaining and patch-clamp recording, we found that Ti3C2Tx MXene promotes NSCs differentiation and neurite growth, increases voltage-gated current of Ca2+ but not Na+ or K+ in matured neurons, boosts their spiking without changing their passive membrane properties, and enhances synaptic transmission between them. Conclusions These results expand our understanding of interaction between Ti3C2Tx MXene and NSCs and provide a critical line of evidence for using Ti3C2Tx MXene in neural interface or scaffold in stem cell therapy.

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