Rhizosphere element circling, multifunctionality, aboveground productivity and trade-offs are better predicted by rhizosphere rare taxa

Microbes, especially abundant microbes in bulk soils, form multiple ecosystem functions, which is relatively well studied. However, the role of rhizosphere microbes, especially rhizosphere rare taxa vs. rhizosphere abundant taxa in regulating the element circling, multifunctionality, aboveground net primary productivity (ANPP) and the trade-offs of multiple functions remains largely unknown. Here, we compared the multiple ecosystem functions, the structure and function of rhizosphere soil bacterial and fungal subcommunities (locally rare, locally abundant, regionally rare, regionally abundant, and entire), and the role of subcommunities in the Zea mays and Sophora davidii sole and Z. mays/S. davidii intercropping ecosystems in subtropical China. Results showed that intercropping altered multiple ecosystem functions individually and simultaneously. Intercropped Z. mays significantly decreased the trade-off intensity compared to sole Z. mays, the trade-off intensity under intercropped S. davidii was significantly higher than under intercropped Z. mays. The beta diversities of bacterial and fungal communities, and fungal functions in each subcommunity significantly differed among groups. Network analysis showed intercropping increased the complexity and positive links of rare bacteria in Z. mays rhizosphere, but decreased the complexity and positive links of rare bacteria in S. davidii rhizosphere and the complexity and positive links of fungi in both intercropped plants rhizosphere. Mantel test showed significant changes in species of locally rare bacteria were most strongly related to nitrogen-cycling multifunctionality, ANPP and trade-offs intensity, significant changes in species of locally rare fungus were most strongly related to carbon-cycling multifunctionality, phosphorus-cycling multifunctionality, and average ecosystem multifunctionality. This research highlights the potential and role of rare rhizosphere microorganisms in predicting and regulating system functions, productivity, and trade-offs.

Automated summary

This study compared the effects of rhizosphere bacterial and fungal subcommunities on element cycling, multifunctionality, aboveground net primary productivity (ANPP), and trade-offs of multiple functions. The results showed that intercropping altered ecosystem functions, with trade-off intensity significantly decreased in intercropped Z. mays compared to sole Z. mays. The diversities and functions of bacterial and fungal communities significantly differed among subcommunities. Species changes in rare bacteria were strongly related to nitrogen-cycling multifunctionality, ANPP, and trade-off intensity, while species changes in rare fungi were strongly related to carbon-cycling and phosphorus-cycling multifunctionality. This research highlights the importance of rare rhizosphere microorganisms in predicting and regulating system functions, productivity, and trade-offs.