CP12 fine-tunes the Calvin-Benson cycle and carbohydrate metabolism in cyanobacteria

Review Biochemical Research Methods

Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production

Xufeng Liu et al.

Summary: This review summarizes the recent progress in engineering cyanobacteria and discusses the achieved and potential utilization of these advancements for the production of the bulk chemical butanol, specifically isobutanol and 1-butanol.

CURRENT OPINION IN BIOTECHNOLOGY (2022)

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Article Biology

Pyruvate:ferredoxin oxidoreductase and low abundant ferredoxins support aerobic photomixotrophic growth in cyanobacteria

Yingying Wang et al.

Summary: The decarboxylation of pyruvate is a central reaction in carbon metabolism. Cyanobacterial PFOR is stable in the presence of oxygen and required for optimal photomixotrophic growth, while the PDH complex is inactivated. Cells shift to utilizing ferredoxin-dependent enzymes under aerobic and highly reducing conditions.

ELIFE (2022)

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Article Biotechnology & Applied Microbiology

GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways

Dennis Schulze et al.

MICROBIAL CELL FACTORIES (2022)

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Article Plant Sciences

The Importance of the C-Terminal Cys Pair of Phosphoribulokinase in Phototrophs in Thioredoxin-Dependent Regulation

Kazuha Fukui et al.

Summary: This study investigates the Trx-mediated regulation of phosphoribulokinase (PRK) in cyanobacteria and Arabidopsis. The results show that the redox status of the C-terminal Cys pair is crucial for activity regulation and complex dissociation/formation in both organisms.

PLANT AND CELL PHYSIOLOGY (2022)

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Article Plant Sciences

First in vivo analysis of the regulatory protein CP12 of the model cyanobacterium Synechocystis PCC 6803: Biotechnological implications

Victoire Blanc-Garin et al.

Summary: This study conducted the first in vivo analysis of the canonical CP12 regulatory protein in the model cyanobacterium Synechocystis PCC 6803 and found that CP12 is essential for the catabolism of exogenously added glucose in the absence of photosynthesis. Additionally, CP12 was found to regulate the redox equilibrium of NADPH, enhancing the production of terpenes.

FRONTIERS IN PLANT SCIENCE (2022)

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Article Plant Sciences

The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low CO2 Shifts in the Cyanobacterium Synechocystis sp. PCC 6803

Stefan Lucius et al.

Summary: Mutants with defects in the glycolytic Entner-Doudoroff (ED) pathway accumulated high levels of glycogen under high CO2 conditions, which were slowly consumed after shifting to low CO2 conditions. This mutation led to a decreased ability to respond to inorganic carbon shifts, hindered growth reactivation in high CO2 conditions, and altered metabolite composition, particularly increasing levels of proline. Deleting eda may promote the utilization of the oxidative pentose-phosphate (OPP) shunt and enhance NADPH levels, suggesting a major regulatory contribution of the ED pathway in mobilizing glycogen reserves during rapid acclimation to fluctuating CO2 conditions.

FRONTIERS IN PLANT SCIENCE (2021)

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Review Plant Sciences

Calvin-Benson cycle regulation is getting complex

Libero Gurrieri et al.

Summary: The complex formation and activation mechanism of two enzymes involved in photosynthesis have been discovered, shedding light on the regulatory potential of photosynthetic carbon fixation. This opens up new avenues for knowledge-based manipulation of the process.

TRENDS IN PLANT SCIENCE (2021)

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Article Plant Sciences

Alterations in the CO2 availability induce alterations in the phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803

Philipp Spaet et al.

Summary: By analyzing the adaptation of cyanobacteria to low CO2 conditions using quantitative phosphoproteomics, it was found that protein abundance changes were closely related to mRNA expression levels, and the functional enrichment of noncorrelating proteins played a crucial role in maintaining cellular homeostasis.

NEW PHYTOLOGIST (2021)

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Article Multidisciplinary Sciences

Red/far-red light signals regulate the activity of the carbon-concentrating mechanism in cyanobacteria

Nadav Oren et al.

Summary: Desiccation-tolerant cyanobacteria are able to survive dehydration cycles, with red/far-red light affecting their inorganic carbon assimilation. The decline in ribulose-1,5-bisphosphate carboxylase/oxygenase activity during desiccation was found to be accelerated by far-red light, negatively impacting Ci affinity in both tolerant and non-tolerant cyanobacteria. It is suggested that red/far-red light perception via phytochromes regulates Ci uptake by cyanobacteria, contributing to desiccation tolerance in certain strains.

SCIENCE ADVANCES (2021)

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Article Biochemistry & Molecular Biology