Journal
FRONTIERS IN MICROBIOLOGY
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.1006138
Keywords
docosahexaenoic acid; comparative transcriptomics; malate; fatty acid metabolism; acetyl-CoA
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Funding
- National Key R&D Program of China
- National Natural Science Foundation of China
- Natural Science Foundation of Fujian Province
- [2021YFA0910501]
- [32101898]
- [31870039]
- [32170069]
- [2022J01635]
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This study analyzed the global changes in gene expression levels of Schizochytrium sp. FJU-512 cultured with malate. The changes mainly affected the genes involved in oxidative phosphorylation, beta-oxidation, and pentose phosphate pathways. By adding malate to the culture medium, the production of docosahexaenoic acid (DHA) increased by 22%.
Schizochytrium is one of the few oleaginous microalgae that produce docosahexaenoic acid (DHA)-rich lipids. In this study, global changes in gene expression levels of Schizochytrium sp. FJU-512 cultured with malate in a 15 l-bioreactor was analyzed using comparative transcriptomics. The changes were found mainly in the genes involved in oxidative phosphorylation, beta-oxidation, and pentose phosphate pathways. Consequently, the global changes in genes associated with the pathways could lead to an increase in the influx throughputs of pyruvate, branched-chain amino acids, fatty acids, and vitamin B6. Our transcriptome analysis indicated pyruvate dehydrogenase E2 component and acetolactate synthase I/II/III large subunit as major contributors to acetyl-CoA biosynthesis, whereas glucose-6-phosphate dehydrogenase was indicated as the major contributor to the biosynthesis of NADPH. An increase in DHA titer of up to 22% was achieved with the addition of malate to the fed-batch culture of Schizochytrium sp. FJU-512. This study provides an alternate method to enhance DHA production in Schizochytrium sp. FJU-512 through malate induced upregulation of genes responsible for acetyl-CoA and NADPH biosynthesis.
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