4.6 Article

Semi-rational engineering membrane binding domain of L-amino acid deaminase from Proteus vulgaris for enhanced a-ketoisocaproate

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Biosynthesis of phenylpyruvic acid from l-phenylalanine using chromosomally engineered Escherichia coli

Tianzhen Xiong et al.

Summary: This study constructed a chromosome engineering strain for whole-cell biotransformation to synthesize phenylpyruvic acid (PPA) from l-phenylalanine. Results showed that increasing the copy number of l-amino acid deaminase (pmLAAD) and deleting the lacI gene could enhance PPA production. The engineered E. coli strain achieved a high PPA titer of 19.14 g/L within 24 hours in a 5-L bioreactor.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2022)

Review Biotechnology & Applied Microbiology

Production of phenylpyruvic acid by engineered l-amino acid deaminase from Proteus mirabilis

Jia Liu et al.

Summary: This study successfully developed an efficient enzymatic strategy for the industrial production of phenylpyruvate from l-phenylpyruvic acid. By modifying the enzyme and based on structural analysis, interactions between the product and enzyme were reduced and enzyme flexibility was increased, leading to improved catalytic efficiency. High production and conversion of PPA were achieved in a 5-L bioreactor.

BIOTECHNOLOGY LETTERS (2022)

Article Chemistry, Physical

Enhanced Catalytic Efficiency of L-amino Acid Deaminase Achieved by a Shorter Hydride Transfer Distance

Yaoyun Wu et al.

Summary: The catalytic efficiency of enzymatic synthesis of alpha-keto acids from alpha-amino acids deamination is limited by the catalytic distance between the substrate and the cofactor. Through protein engineering strategy, two variants were developed to shorten the distance and improve the efficiency of the process. The modified variants showed improved substrate binding pose and led to successful conversion of selected substrates with high yields.

CHEMCATCHEM (2021)

Article Biotechnology & Applied Microbiology

Efficient production of phenylpropionic acids by an amino-group-transformation biocatalytic cascade

Jinhui Wang et al.

BIOTECHNOLOGY AND BIOENGINEERING (2020)

Article Biotechnology & Applied Microbiology

Efficient enzymatic synthesis of α-keto acids by redesigned substrate-binding pocket of the L-amino acid deaminase (PmiLAAD)

Licheng Wu et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Review Biochemistry & Molecular Biology

Identification and development of amino acid oxidases

Yasuhisa Asano et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2019)

Review Agricultural Engineering

Biotechnological production of alpha-keto acids: Current status and perspectives

Yang Song et al.

BIORESOURCE TECHNOLOGY (2016)

Article Chemistry, Physical

ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB

James A. Maier et al.

JOURNAL OF CHEMICAL THEORY AND COMPUTATION (2015)

Article Biochemistry & Molecular Biology

Discovery of novel membrane binding structures and functions

Irina Kufareva et al.

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE (2014)

Article Biochemistry & Molecular Biology

Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus

Patrick M. McTernan et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Review Chemistry, Multidisciplinary

The Amber biomolecular simulation programs

DA Case et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2005)

Article Biochemistry & Molecular Biology

H++:: a server for estimating pKas and adding missing hydrogens to macromolecules

JC Gordon et al.

NUCLEIC ACIDS RESEARCH (2005)