Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.960507
Keywords
metavirome analysis; bat; virome; viral isolation and identification; phylogenetic analysis
Categories
Funding
- National Natural Science Foundation of China
- Natural Science Foundation of Zhejiang Province
- Science and Technology Project of Wenzhou, Zhejiang, China
- [32002312]
- [LQ21H160001]
- [Y20210080]
- [Y2020103]
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This study gathered Rhinolophus sinicus samples and used metavirome techniques to obtain enriched viral sequences of the Rhinolophus sinicus-specific virome. Chikungunya virus (CHIKV) and Japanese encephalitis virus (JEV) present in Rhinolophus sinicus were validated through PCR. Notably, it was the first time JEV was isolated from Rhinolophus sinicus. The newly isolated JEV sequence showed increased infectivity during passaging in Vero cells.
To exploit the Rhinolophus sinicus-specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in Rhinolophus sinicus. The newly isolated JEV-China/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in Rhinolophus sinicus and reveals new light on the ecology of other important viral hosts.
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