Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2022.1012105
Keywords
virulent shigella sonnei; multiplex cross displacement amplification; lateral flow biosensor; sensitivity; specificity
Categories
Funding
- Basic Research Project of Shanxi Province
- [2015011115]
- [2018RU010]
Ask authors/readers for more resources
In this study, a multiplex MCDA-LFB assay was developed for rapid and convenient identification of virulent S. sonnei within 30 minutes, without the need for expensive lab equipment.
Shigella sonnei is the most common Shigella spp. in developed areas and the second most common in undeveloped regions. In this study, a multiple cross displacement amplification (MCDA) assay was used in combination with a lateral flow biosensor (LFB) assay to detect virulent S. sonnei strains containing the ipaH and wbgX genes. The multiplex MCDA-LFB assay detected wbgX at >= 1 pg/mu L and ipaH at >= 10 fg/mu L within 30 min in pure cultures maintained at 63 degrees C. This assay was sensitive for similar to 37 CFU of virulent S. sonnei and similar to 3.7 CFU of Shigella spp. and enteroinvasive E. coli in stimulated fecal samples and had 100% specificity among 59 reference strains. The MCDA-LFB assay was also able to differentiate between virulent S. sonnei and other Shigella spp. and enteroinvasive E. coli among 99 clinical isolates. In summary, a multiplex MCDA-LFB assay was developed for rapid, convenient, point-of-care, and accurate identification of virulent S. sonnei within 30 min and at a constant temperature without the need for expensive lab equipment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available