Journal
ELIFE
Volume 11, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.77327
Keywords
sick sinus syndrome; sinus arrest; Dnajb6; electrocardiogram; genetic diseases; zebrafish; Zebrafish
Categories
Funding
- Mayo Foundation
- Science and Technology Innovation Action Plan of Shanghai [201409005600]
- NIH [GM063904, R01HL141214, R00HL138160, R01HL131517, P01HL141084, 1OT2OD026580-01, R44OD024874, R01HL142704]
- American Heart Association [16SDG29120011, 17POST33370089, 846898]
- Wisconsin Partnership Program [4140]
- UC Davis School of Medicine Dean's Fellow Award
- American Heart Association Scientist Development Award [15SDG24910015]
- American Heart Association Postdoctoral Fellowship [20POST35120462]
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The study utilized a gene-break transposon (GBT) library to identify a novel causative gene Dnajb6 for sick sinus syndrome, revealing the heterogeneity of cardiac pacemaker cells.
Previously we showed the generation of a protein trap library made with the gene-break transposon (GBT) in zebrafish (Danio rerio) that could be used to facilitate novel functional genome annotation towards understanding molecular underpinnings of human diseases (Ichino et al, 2020). Here, we report a significant application of this library for discovering essential genes for heart rhythm disorders such as sick sinus syndrome (SSS). SSS is a group of heart rhythm disorders caused by malfunction of the sinus node, the heart's primary pacemaker. Partially owing to its aging-associated phenotypic manifestation and low expressivity, molecular mechanisms of SSS remain difficult to decipher. From 609 GBT lines screened, we generated a collection of 35 zebrafish insertional cardiac (ZIC) mutants in which each mutant traps a gene with cardiac expression. We further employed electrocardiographic measurements to screen these 35 ZIC lines and identified three GBT mutants with SSS-like phenotypes. More detailed functional studies on one of the arrhythmogenic mutants, GBT411, in both zebrafish and mouse models unveiled Dnajb6 as a novel SSS causative gene with a unique expression pattern within the subpopulation of sinus node pacemaker cells that partially overlaps with the expression of hyperpolarization activated cyclic nucleotide gated channel 4 (HCN4), supporting heterogeneity of the cardiac pacemaker cells.
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