Journal
CLINICAL EPIGENETICS
Volume 14, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13148-022-01334-3
Keywords
Epigenetics; DNA methylation; ddPCR; Plasma; Circulating DNA; BCAT1; Lung cancer; Noninvasive
Categories
Funding
- ISCIII [PI19/00572, PI19/00230]
- European Union ERDF, A way to make Europe
- Agencia Valenciana de Innovacion AVI [INNVA1/2020/71]
- Generalitat Valenciana [FDEGENT/2019/014]
Ask authors/readers for more resources
This study validates a noninvasive detection method using a 4-gene signature of DNA methylation levels for efficient diagnosis of lung cancer in plasma.
Lung cancer patients are diagnosed at late stages when curative treatments are no longer possible; thus, molecular biomarkers for noninvasive detection are urgently needed. In this sense, we previously identified and validated an epigenetic 4-gene signature that yielded a high diagnostic performance in tissue and invasive pulmonary fluids. We analyzed DNA methylation levels using the ultrasensitive digital droplet PCR in noninvasive samples in a cohort of 83 patients. We demonstrated that BCAT1 is the candidate that achieves high diagnostic efficacy in circulating DNA derived from plasma (area under the curve: 0.85). Impact of potentially confounding variables was also explored.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available