Realized Heritability, Risk Assessment, and Inheritance Pattern in Earias vittella (Lepidoptera: Noctuidae) Resistant to Dipel (Bacillus thuringiensis Kurstaki)

Earias vittella Fabricius is a potential cotton and okra pest in South Asia. The realized heritability, risk assessment, and inheritance mode of Bacillus thuringiensis Kurstaki (Btk) resistance were determined in the Dipel-selected (DIPEL-SEL) E. vittella. The DIPEL-SEL strain had a 127.56-fold rise in Dipel resistance after nine generations compared to the laboratory reference strain (LAB-PK). The overlapping of 95% fiducial limits in the median lethal concentrations (LC(50)s) of the F-1 (DIPEL-SEL male x LAB-PK female) and F-1(double dagger) (DIPEL-SEL female x LAB-PK male) suggested a lack of sex linkage and an autosomal Dipel resistance. The dominance (D-LC) values for the F-1 (0.86) and F-1(double dagger) (0.94) indicated incompletely dominant resistance to Dipel. Backcrossing of the F-1 female x Lab-PK male revealed a polygenic response of resistance to Dipel. The realized heritability estimation (h(2)) of resistance to Dipel was 0.19. With 20% to 90% selection mortality, the generations required for a tenfold increase in LC50 of Dipel were 4.7-22.8, 3.1-14.9, and 2.3-11.1 at h 2 of 0.19, 0.29, and 0.39, respectively, and a constant slope of 1.56. At slope values of 2.56 and 3.56 with a constant h(2) = 0.19, 7.7-37.4 and 10.6-52.0 generations were needed to increase the tenfold LC50 of Dipel in the DIPEL-SEL E. vittella. It is concluded that the DIPEL-SEL E. vittella has an autosomal, incompletely dominant, and polygenic nature of resistance. The h(2) of 0.19 suggested that a high proportion of phenotypic variation for the Dipel resistance in E. vittella was heritable genetic variation. The present results will support the creation of an effective and suitable resistance management plan for better control of E. vittella.

Automated summary

The study determined the genetic characteristics of Bacillus thuringiensis Kurstaki (Btk) resistance in Earias vittella Fabricius, a potential pest for cotton and okra in South Asia. It was found that the resistance had autosomal, incompletely dominant, and polygenic nature. The study also showed that a high proportion of the resistance variation was heritable. These findings will support the development of effective resistance management plans to control E. vittella.