Anabaenopeptins from Nostoc edaphicum CCNP1411

Cyanobacteria of the Nostoc genus belong to the most prolific sources of bioactive metabolites. In our previous study on Nostoc edaphicum strain CCNP1411, the occurrence of cyanopeptolins and nostocyclopeptides was documented. In the current work, the production of anabaenopeptins (APs) by the strain was studied using genetic and chemical methods. Compatibility between the analysis of the apt gene cluster and the structure of the identified APs was found. Three of the APs, including two new variants, were isolated as pure compounds and tested against four serine proteases and carboxypeptidase A (CPA). The in vitro enzymatic assays showed a typical activity of this class of cyanopeptides, i.e., the most pronounced effects were observed in the case of CPA. The activity of the detected compounds against important metabolic enzymes confirms the pharmaceutical potential of anabaenopeptins.

Automated summary

Cyanobacteria of the Nostoc genus are prolific sources of bioactive metabolites, and previous studies have documented the presence of cyanopeptolins and nostocyclopeptides in Nostoc edaphicum strain CCNP1411. This study investigates the production of anabaenopeptins (APs) by the strain using genetic and chemical methods. The results show that the identified APs are structurally compatible with the analysis of the apt gene cluster, and three APs, including two new variants, were isolated and tested against various enzymes. The in vitro assays confirm the pharmaceutical potential of anabaenopeptins, especially against carboxypeptidase A.