Journal
GREENHOUSE GASES-SCIENCE AND TECHNOLOGY
Volume 12, Issue 5, Pages 661-668Publisher
WILEY PERIODICALS, INC
DOI: 10.1002/ghg.2179
Keywords
cultivation; fermentation; M; trichosporium; methanol; methanotroph
Funding
- Universiti Kebangsaan Malaysia (UKM) [UK-GUP-2013-031]
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The research focused on the growth characteristics and optimal conditions of the methanotroph Methylosinus trichosporium for methanol production, indicating that the bacterium can maximize methanol production under specific methane-air ratio, pH, and phosphate ion concentration.
The technology of methanol production from the fermentation of methanol-producing bacteria (methanotroph) is still in the research phase. Maximizing the methanol production through bacteria fermentation in this process is a challenge to researchers. By optimizing the biological process, this technology can potentially fill the demand gap in methanol industries. Methylosinus trichosporium is a methanotroph that is used for the production of methanol in this analysis. Using OD600 analysis, the rate and growth profile for M. trichosporium is determined. The one factor at a time (OFAT) method was chosen to optimize the methanol production (percentage of methane, pH, and phosphate ion), and the optimum condition for this bacterium was determined. M. trichosporium showed a specific growth rate at 0.02 h(-1) with the exponential phase reaching the maximum at 90 h of the cultivation period. Optimization analysis showed that the maximum methanol production by this bacterium was at a 1:1 methane-air ratio, a pH of 6.3-6.5, and 40 mM of phosphate ions. The ability of M. trichosporium to produce methanol was proved in this analysis with the maximum rate of production at 0.072 mg L-1 h(-1). (c) 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
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