Intergenerational effects of overfeeding on aversive learning in zebrafish (Danio rerio)

The obesity epidemic is concerning as obesity appears to negatively impact cognition and behavior. Furthermore, some studies suggest that this negative effect could be carried across generations from both mothers and fathers although evidence is not consistent. Here, we attempt to address how obesogenic diets in the parental generation (F0) can impact offspring's cognition and anxiety intergenerationally (F1) in a zebrafish model. We compare both mean trait values and their variances. Using a multifactorial design, we created a total of four groups: F1T (treatment mothers x treatment fathers); F1M (treatment mothers x control fathers); F1P (treatment fathers x control mothers); and F1C (control mothers x control fathers, F1C); and subjected them to anxiety tank tests and aversive learning assays. When both parents were exposed, offspring (F1T) displayed the poorest aversive learning, while offspring that only had one parent exposed (F1P and F1M) learnt the aversive learning task the best. Zebrafish in all groups displayed no statistically significant differences in anxiety-associated behaviors. Males and females also performed similarly in both anxiety and aversive learning assays. While all F1 groups had similar levels of fasting blood glucose, variance in glucose levels were reduced in F1P and F1T indicating the importance of investigating heteroskedasticity between groups. Furthermore, anxiety behaviors of these two groups appeared to be less repeatable. To our knowledge, this is the first study to test the intergenerational effects of an obesogenic diet on zebrafish cognition. Our multifactorial design as well as repeated tests also allowed us to disentangle maternal and paternal effects (as well as combined effects) and accurately detect subtle information such as between-individual variation.

Automated summary

The obesity epidemic and its impact on cognition and behavior is a cause for concern. This study investigates the intergenerational effects of obesogenic diets on zebrafish cognition and anxiety. Results suggest that the negative effects of obesity on aversive learning can be carried across generations, with offspring from both obese parents performing poorly. However, offspring with only one obese parent performed the best. There were no significant differences in anxiety behaviors between groups. This study highlights the importance of investigating the effects of obesity on cognitive function and provides insights into intergenerational effects.