Journal
CELL REPORTS
Volume 40, Issue 13, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2022.111408
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Funding
- Blavatnik Family Foundation Fellowship
- G Harold and Leila Y Mathers Foundation
- NIH [T32GM008275, F31AG060672, R01GM099836, R21AG061784, R01GM138690]
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The high-resolution structure of Skd3-substrate complex is reported, revealing the mechanism of Skd3 protein disaggregation and its association with disease inheritance patterns.
The AAA+ protein, Skd3 (human CLPB), solubilizes proteins in the mitochondrial intermembrane space, which is critical for human health. Skd3 variants with defective protein-disaggregase activity cause severe congenital neutropenia (SCN) and 3-methylglutaconic aciduria type 7 (MGCA7). How Skd3 disaggregates proteins remains poorly understood. Here, we report a high-resolution structure of a Skd3-substrate complex. Skd3 adopts a spiral hexameric arrangement that engages substrate via pore-loop interactions in the nucleotide-binding domain (NBD). Substrate-bound Skd3 hexamers stack head-to-head via unique, adaptable ankyrin-repeat domain (ANK)-mediated interactions to form dodecamers. Deleting the ANK linker region reduces dodecamerization and disaggregase activity. We elucidate apomorphic features of the Skd3 NBD and C-terminal domain that regulate disaggregase activity. We also define how Skd3 subunits collaborate to disaggregate proteins. Importantly, SCN-linked subunits sharply inhibit disaggregase activity, whereas MGCA7-linked subunits do not. These advances illuminate Skd3 structure and mechanism, explain SCN and MGCA7 inheritance patterns, and suggest therapeutic strategies.
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