Structural characterization and antibacterial activity of silver nanoparticles synthesized using a low-molecular-weight Royal Jelly extract

In recent years silver nanoparticles (Ag NPs) gained increased and widespread applications in various fields of industry, technology, and medicine. This study describes the green synthesis of silver nanoparticles (Ag NPs) applying a low-molecular-weight fraction (LMF) of Royal Jelly, the nanoparticle characterization, and particularly their antibacterial activity. The optical properties of NPs, characterized by UV-Vis absorption spectroscopy, showed a peak at similar to 430 nm. The hydrodynamic radius and concentration were determined by complementary dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The particle morphology was investigated using transmission electron microscopy (TEM), and the crystallinity of the silver was confirmed by X-ray diffraction (XRD). The antibacterial activities were evaluated utilizing Gram-negative and Gram-positive bacteria and colony counting assays. The growth inhibition curve method was applied to obtain information about the corresponding minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) required. Obtained results showed that (i) the sizes of Ag NPs are increasing within the increase of silver ion precursor concentration, (ii) DLS, in agreement with NTA, showed that most particles have dimensions in the range of 50-100 nm; (iii) E. coli was more susceptible to all Ag NP samples compared to B. subtilis.

Automated summary

In recent years, silver nanoparticles have been widely used in various fields such as industry, technology, and medicine. This study focused on the green synthesis of silver nanoparticles using a low-molecular-weight fraction of Royal Jelly and investigated their antibacterial activity. The results showed that the size of the silver nanoparticles increased with the increase in silver ion precursor concentration, and most particles had dimensions in the range of 50-100 nm. Additionally, E. coli was more susceptible to the silver nanoparticles compared to B. subtilis.