Journal
SCIENTIFIC REPORTS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-22256-3
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Funding
- ministere de l'Enseignement superieur, de la Recherche et de l'Innovation (MESRI)
- Investissement d'Avenir grant (CEBA) [ANR-10-LABX-0025]
- Agence Nationale de la Recherche (ANR) [ANR-10-LABX-0025] Funding Source: Agence Nationale de la Recherche (ANR)
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This study explored the metabolites of termite mutualistic strains from French Guiana using metabolomic analysis and whole genome sequencing. Two families of chlorinated polyketides were identified, and the biosynthetic pathways related to these compounds were defined. New halogenated metabolites were synthesized by exploiting the enzymatic promiscuity.
We gathered a collection of termite mutualistic strains from French Guiana to explore the metabolites of symbiotic microorganisms. Molecular networks reconstructed from a metabolomic analysis using LC-ESI-MS/MS methodology led us to identify two families of chlorinated polyketides, i.e., azaphilones from Penicillium sclerotiorum and ilicicolins from Neonectria discophora. To define the biosynthetic pathways related to these two types of scaffolds, we used a whole genome sequencing approach followed by hybrid assembly from short and long reads. We found two biosynthetic gene clusters, including two FAD-dependent halogenases. To exploit the enzymatic promiscuity of the two identified FAD halogenases, we sought to biosynthesize novel halogenated metabolites. An OSMAC strategy was used and resulted in the production of brominated analogs of ilicicolins and azaphilones as well as iodinated analogs of azaphilones.
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