Live imaging of delamination in Drosophila shows epithelial cell motility and invasiveness are independently regulated

Delaminating cells undergo complex, precisely regulated changes in cell-cell adhesion, motility, polarity, invasiveness, and other cellular properties. Delamination occurs during development and in pathogenic conditions such as cancer metastasis. We analyzed the requirements for epithelial delamination in Drosophila ovary border cells, which detach from the structured epithelial layer and begin to migrate collectively. We used live imaging to examine cellular dynamics, particularly epithelial cells' acquisition of motility and invasiveness, in delamination-defective mutants during the time period in which delamination occurs in the wild-type ovary. We found that border cells in slow border cells (slbo), a delamination-defective mutant, lacked invasive cellular protrusions but acquired basic cellular motility, while JAK/STAT-inhibited border cells lost both invasiveness and motility. Our results indicate that invasiveness and motility, which are cooperatively required for delamination, are regulated independently. Our reconstruction experiments also showed that motility is not a prerequisite for acquiring invasiveness.

Automated summary

Delaminating cells undergo complex, precisely regulated changes in cell-cell adhesion, motility, polarity, invasiveness, and other cellular properties. In the study of epithelial delamination in Drosophila ovary border cells, it was found that invasiveness and motility are cooperatively required for delamination and regulated independently. Additionally, the acquisition of invasiveness does not depend on the prerequisite of motility.