4.7 Article

Protein delivery to living cells by thermal stimulation for biophysical investigation

Related references

Note: Only part of the references are listed.
Review Chemistry, Multidisciplinary

Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR

Enrico Luchinat et al.

Summary: A detailed understanding of the complex processes that contribute to the functionality of cells and organisms is crucial for disease comprehension and drug development. Among the available structural techniques, solution NMR is the only method capable of investigating macromolecular structures and dynamic behaviors directly within living cells. Over the past 20 years, in-cell NMR has emerged as a powerful tool, allowing researchers to study cell samples, isotopic labeling, and bioreactor devices, thus enhancing the lifetime of cells for real-time monitoring of intracellular metabolites and proteins.

CHEMICAL REVIEWS (2022)

Article Biology

Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy

Nicholas Sciolino et al.

Summary: This study demonstrates a new method for in-cell NMR using microfluidics to deliver labeled target proteins to cells for high-resolution structural studies. This technique does not affect cell viability and allows for long-term eukaryotic in-cell NMR experiments.

COMMUNICATIONS BIOLOGY (2022)

Review Chemistry, Multidisciplinary

Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR

Enrico Luchinat et al.

Summary: Understanding the complex processes that keep cells and organisms alive is essential for comprehending diseases and developing new drugs. Solution NMR is a key technique to investigate the structure and dynamic behavior of biological macromolecules within living cells at high resolution.

CHEMICAL REVIEWS (2022)

Article Chemistry, Physical

Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance Measurements In-Cell

Zikri Hasanbasri et al.

Summary: Sensitive in-cell distance measurements in proteins can be achieved using deuterated OX063 with a maleimide linker (mOX063-d(24)), enabling high protein labeling and more sensitive measurements at 150K compared to 80K. The signal intensity of mOX063-d(24) remains appreciable even in the crowded environment of cells.

JOURNAL OF PHYSICAL CHEMISTRY B (2021)

Article Chemistry, Physical

Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins: CuII-CuII and Nitroxide-Nitroxide Cases

Katrin Ackermann et al.

Summary: The study utilizes biophysical methods such as pulse dipolar electron paramagnetic resonance spectroscopy to investigate complex biomolecular assemblies implicated in human health and disease, demonstrating the feasibility of PDS measurements at sub-micromolar concentrations and highlighting the significance for applications with concentration limitations.

JOURNAL OF PHYSICAL CHEMISTRY B (2021)

Article Chemistry, Physical

In-Cell Double Electron-Electron Resonance at Nanomolar Protein Concentrations

Svetlana Kucher et al.

Summary: The study demonstrates the feasibility of using double electron-electron resonance (DEER) technique to measure distances between protein sites at very low protein concentrations, providing a new approach for studying conformational changes of biomolecules.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2021)

Article Biochemistry & Molecular Biology

Rapid protein delivery to living cells for biomolecular investigation

Francesco Torricella et al.

Summary: The innovative thermal vesiculation method offers a simple and efficient approach for delivering proteins into both prokaryotic and eukaryotic cells, allowing for the internalization of proteins with different molecular weights and conformations without compromising their structural properties and cell viability. This versatile method opens up the possibility for scientists to study proteins directly in living cells through a wide range of techniques.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021)

Review Biochemistry & Molecular Biology

Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies

F. Torricella et al.

Summary: SDSL-EPR technique involves attaching a paramagnetic label to specific positions of proteins for EPR spectroscopic study, providing information on local conformational dynamics, distances between spin centers, structures, and functions. The versatility of this spectroscopic approach in protein structural characterization has been demonstrated through recent studies, aiming to spread its applicability in various fields of structural biology, particularly for non-experts.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2021)

Review Biochemistry & Molecular Biology

In-Cell EPR: Progress towards Structural Studies Inside Cells

Alessio Bonucci et al.

CHEMBIOCHEM (2020)

Article Chemistry, Multidisciplinary

Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency

Enrico Luchinat et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Chemistry, Physical

In-Cell Trityl-Trityl Distance Measurements on Proteins

Yin Yang et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2020)

Article Chemistry, Multidisciplinary

Compaction of RNA Duplexes in the Cell

Alberto Collauto et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Article Multidisciplinary Sciences

In-cell destabilization of a homodimeric protein complex detected by DEER spectroscopy

Yin Yang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Chemistry, Physical

Combining site-directed spin labeling in vivo and in-cell EPR distance determination

Pia Widder et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2020)

Article Chemistry, Physical

In-Cell EPR Distance Measurements on Ubiquitin Labeled with a Rigid PyMTA-Gd(III) Tag

Yin Yang et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2019)

Review Medicine, Research & Experimental

Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers

Yi-Wei Lee et al.

THERANOSTICS (2019)

Review Chemistry, Multidisciplinary

In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation

Enrico Luchinat et al.

ACCOUNTS OF CHEMICAL RESEARCH (2018)

Article Chemistry, Multidisciplinary

A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies

Ganesan Karthikeyan et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Chemistry, Physical

High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label

Yin Yang et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2018)

Review Chemistry, Multidisciplinary

In-cell NMR: a topical review

Enrico Luchinat et al.

IUCRJ (2017)

Article Chemistry, Physical

A New Wavelet Denoising Method for Experimental Time-Domain Signals: Pulsed Dipolar Electron Spin Resonance

Madhur Srivastava et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2017)

Article Multidisciplinary Sciences

Structural disorder of monomeric α-synuclein persists in mammalian cells

Francois-Xavier Theillet et al.

NATURE (2016)

Article Biochemistry & Molecular Biology

Strategies for Protein NMR in Escherichia coli

Guohua Xu et al.

BIOCHEMISTRY (2014)

Article Chemistry, Multidisciplinary

Probing Protein Conformation in Cells by EPR Distance Measurements using Gd3+ Spin Labeling

Andrea Martorana et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Article Multidisciplinary Sciences

Residue level quantification of protein stability in living cells

William B. Monteith et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Biochemistry & Molecular Biology

Visualization of Redox-Controlled Protein Fold in Living Cells

Lucia Banci et al.

CHEMISTRY & BIOLOGY (2013)

Article Biochemical Research Methods

The global analysis of DEER data

Suzanne Brandon et al.

JOURNAL OF MAGNETIC RESONANCE (2012)

Article Biochemical Research Methods

NIH Image to ImageJ: 25 years of image analysis

Caroline A. Schneider et al.

NATURE METHODS (2012)

Article Chemistry, Physical

Rotamer libraries of spin labelled cysteines for protein studies

Yevhen Polyhach et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2011)

Article Chemistry, Multidisciplinary

Nanoparticle-Mediated Cytoplasmic Delivery of Proteins To Target Cellular Machinery

Shyam Sundhar Bale et al.

ACS NANO (2010)

Article Multidisciplinary Sciences

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

Kohsuke Inomata et al.

NATURE (2009)

Article Multidisciplinary Sciences

In-Cell Biochemistry Using NMR Spectroscopy

David S. Burz et al.

PLOS ONE (2008)

Article Multidisciplinary Sciences

Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes

Philipp Selenko et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Multidisciplinary Sciences

FlgM gains structure in living cells

MM Dedmon et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)

Article Multidisciplinary Sciences

Expanding the genetic code of Escherichia coli

L Wang et al.

SCIENCE (2001)

Article Biochemical Research Methods

Dead-time free measurement of dipole-dipole interactions between electron spins

M Pannier et al.

JOURNAL OF MAGNETIC RESONANCE (2000)