Journal
JOURNAL OF MATERIALS RESEARCH
Volume 31, Issue 3, Pages 321-327Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1557/jmr.2015.406
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Funding
- NIH [2R01 DE016849]
- CTSI-SOS Grant [000166]
- S10 Shared Instrumentation Grant [S10RR026645]
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Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. A Dspp knockout (Dspp(-/-)) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the polymer-induced liquid-precursor (PILP) system can restore its mechanical properties. Dentin from six-week old Dspp(-/-) and wild-type mice was treated with CaP solution containing poly(ASP) for up to 14 days. Elastic modulus and hardness before and after treatment were correlated with mineralization from Micro x-ray computed tomography (Micro-XCT). Transmission electron microscopy (TEM)/Selected area electron diffraction (SAED) were used to compare matrix mineralization and crystallography. Mechanical properties of the Dspp(-/-) dentin were significantly less than wild-type dentin and recovered significantly (P<0.05) after PILP-treatment, reaching values comparable to wild-type dentin. Micro-XCT showed mineral recovery similar to wild-type dentin after PILP-treatment. TEM/SAED showed repair of patchy mineralization and complete mineralization of defective dentin. This approach may lead to new strategies for hard tissue repair.
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