Journal
NUTRIENTS
Volume 14, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/nu14193980
Keywords
A; tenuissimum flower flavonoids; network pharmacology; molecular docking; gut microbiota; type 2 diabetes mellitus
Categories
Funding
- National Natural Science Foundation of China [31570348]
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This study found that flavonoids-rich A. tenuissimum flower can effectively inhibit α-glucosidase activity, improving glycolipid metabolic disorders and inflammation in type 2 diabetes patients.
Forty compounds were isolated and characterized from A. tenuissimum flower. Among them, twelve flavonoids showed higher alpha-glucosidase inhibition activities in vitro than acarbose, especially kaempferol. The molecular docking results showed that the binding of kaempferol to alpha-glucosidase (GAA) could reduce the hydrolysis of substrates by GAA and reduce the glucose produced by hydrolysis, thus exhibiting alpha-glucosidase inhibition activities. The in vivo experiment results showed that flavonoids-rich A. tenuissimum flower could decrease blood glucose and reduce lipid accumulation. The protein expression levels of RAC-alpha serine/threonine-protein kinase (AKT1), peroxisome proliferator activated receptor gamma (PPARG), and prostaglandin G/H synthase 2 (PTGS2) in liver tissue were increased. In addition, the Firmicutes/Bacteroidetes (F/B) ratio was increased, the level of gut probiotics Bifidobacterium was increased, and the levels of Enterobacteriaceae and Staphylococcus were decreased. The carbohydrate metabolism, lipid metabolism, and other pathways related to type 2 diabetes mellitus were activated. This study indicating flavonoids-rich A. tenuissimum flower could improve glycolipid metabolic disorders and inflammation in diabetic mice by modulating the protein expression and gut microbiota.
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