Journal
NATURE COMMUNICATIONS
Volume 13, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-32766-3
Keywords
-
Categories
Funding
- Genomics Core Facility of the Case Western Reserve University School of Medicine's Genetics and Genome Sciences Department
- National Institutes of Health [GM118018, GM125086, R35NS116842]
- Bloomberg Philanthropies
- Single cell and Transcriptomics core of Johns Hopkins University School of Medicine
Ask authors/readers for more resources
Mutations in tRNA processing factors can result in myelin disorders, particularly in oligodendrocytes. This study investigates the differences in tRNA modifications and mRNA decay between differentiated oligodendrocytes and precursor cells. The findings suggest that tRNA biology may play a crucial role in oligodendrocyte function, and disruptions in tRNA metabolism can contribute to leukodystrophies and white matter disease.
Mutations in tRNA processing factors can lead to myelin disorders. This study shows that differentiated oligodendrocytes, cells that make the myelin, are characterized by different tRNA modifications and mRNA decay compared to their precursor cells. Oligodendrocytes are specialized cells that confer neuronal myelination in the central nervous system. Leukodystrophies associated with oligodendrocyte deficits and hypomyelination are known to result when a number of tRNA metabolism genes are mutated. Thus, for unknown reasons, oligodendrocytes may be hypersensitive to perturbations in tRNA biology. In this study, we survey the tRNA transcriptome in the murine oligodendrocyte cell lineage and find that specific tRNAs are hypomodified in oligodendrocytes within or near the anticodon compared to oligodendrocyte progenitor cells (OPCs). This hypomodified state may be the result of differential expression of key modification enzymes during oligodendrocyte differentiation. Moreover, we observe a concomitant relationship between tRNA hypomodification and tRNA decoding potential; observing oligodendrocyte specific alterations in codon optimality-mediated mRNA decay and ribosome transit. Our results reveal that oligodendrocytes naturally maintain a delicate, hypersensitized tRNA/mRNA axis. We suggest this axis is a potential mediator of pathology in leukodystrophies and white matter disease when further insult to tRNA metabolism is introduced.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available