Journal
EPIGENOMICS
Volume 14, Issue 17, Pages 987-994Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2022-0180
Keywords
association; choline; genetic variants; orofacial clefts; PEMT
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Funding
- FONDECYT [1170805]
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This study assessed the association between PEMT variants and nonsyndromic cleft lip with or without cleft palate in Chile, as well as the effects of these variants on global DNA methylation. The study found that the variants rs7649 and rs4646409 increased the risk of nonsyndromic cleft lip with or without cleft palate in the Chilean population and led to a decrease in DNA methylation. This suggests that this gene may be involved in this birth defect.
Aim: To assess the association between PEMT variants and nonsyndromic cleft lip with or without cleft palate in Chile and the effects of these variants on global DNA methylation. Subjects & methods: The authors obtained genotypes for nine variants from 247 cases and 453 controls for genotype-phenotype associations. The effect of significant polymorphisms on global DNA methylation (percentage of long interspersed element-1 methylation) was evaluated in a subsample of 95 controls. Results: After multiple comparison corrections, variants rs7649 and rs4646409 were associated with nonsyndromic cleft lip with or without cleft palate. Carriers of risk alleles presented lower DNA methylation levels than noncarriers. Conclusion: According to functional analysis for risk variants from previous reports, the authors infer that a decrease of methyl group availability is occurring in affected subjects. Plain language summary This study evaluated if variants in the gene named PEMT confers an increased risk for nonsyndromic cleft lip with or without cleft palate in Chile and its possible effects on methylation of DNA, a variable linked to gene expression modulation. The study found that the variants recognized as rs7649 and rs4646409 increase the risk of nonsyndromic cleft lip with or without cleft palate in the Chilean population and decrease DNA methylation. The authors conclude that this gene may be involved in this birth defect. New studies are needed to confirm the relation between this condition and DNA methylation mediated by these genetic variants.
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