4.7 Article

Influence of supplementation of probiotic bacteria Lactobacillus plantarum and Lactobacillus curvatus on selected parameters of liver iron metabolism in rats on high-fat iron-deficient diet

Journal

JOURNAL OF FUNCTIONAL FOODS
Volume 96, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.jff.2022.105205

Keywords

Probiotics; Iron; Minerals; Iron deficiency; Hemojuvelin; Gene expression

Funding

  1. National Science Centre, Poland [2019/33/N/NZ9/00699]
  2. Department of Food Science and Nutrition, Poznan University of Life Sciences, Poznan, Poland [506.786.03.00]

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Supplementation with Lactobacillus plantarum and Lactobacillus curvatus increased serum ferroportin concentration in rats on a high-fat iron-deficient diet, and Lactobacillus curvatus enhanced liver expression of the Tfrc gene.
Background: Iron deficiency constitutes the most frequent diet deficit. A high-fat diet also leads to disturbances in iron metabolism. There is evidence that probiotics favorably influence iron turnover. Purpose: To compare the influence of supplementation with probiotic Lactobacillus plantarum and Lactobacillus curvatus with and without iron supply on liver iron metabolism in rats on high-fat iron-deficient diet. Methods: In the first stage (8 weeks), rats were fed a control diet (group C, n = 8), high-fat diet (group HF, n = 8), or high-fat iron-deficient diet (group HFDEF, n = 48). In the second stage (8 weeks), the rats in the C and HF groups were kept on the same diets, while the rats in the HFDEF group were divided into 6 groups (n = 8 each) and fed as follows: group HFDEF-high-fat iron-deficient diet, group HFDEFFe-high-fat iron-deficient diet with iron supplementation, groups HFDEFLp and HFDEFLc-high-fat iron-deficient diet with L. plantarum or L. curvatus supplementation, respectively, and groups HFDEFFeLp and HFDEFFeLc-high-fat iron-deficient diet supplemented with iron and L. plantarum or L. curvatus, respectively. The rats were subsequently sacrificed, and their serum and liver samples were collected for analysis. Results: Serum ferroportin level was higher in the HFDEFFe, HFDEFLp, HFDEFLc, and HFDEFFeLp groups than in the C and HFDEF groups. The hepatic expression of type 1 transferrin receptor gene (Tfrc) was higher in the HFDEFFeLc group than in the C group. No differences in serum iron, hepcidin, hemojuvelin, transferrin, and ferritin levels and in hepatic type 2 transferrin receptor gene expression were observed between the groups. Conclusions: In rats on high-fat iron-deficient diet, the supplementation of L. curvatus and L. plantarum increased serum ferroportin concentration, and L. curvatus enhanced liver expression of the Tfrc gene.

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