Application of a new volumetric microsampling device for quantitative bioanalysis of immunosuppression

Background: Volumetric absorptive microsampling may reduce the blood collection burden associated with therapeutic drug monitoring of immunosuppression to prevent organ transplant rejection. This work describes the development of a laboratory and analytical technique for quantifying tacrolimus and mycophenolic acid (MPA) from the Tasso-M20 (TM) in human whole blood using bead-based impact-assisted extraction. Results: The sampled blood volume was accurate with estimated volumes within Conclusion: The sampling and extraction procedures allowed for accurate quantification of tacrolimus and MPA. Exploration of abuse scenarios identified important education points for patients conducting home-based sample collections in the future.

Automated summary

This study describes the development of a laboratory and analytical technique for quantifying tacrolimus and mycophenolic acid from Tasso-M20 in human whole blood. The sampling and extraction procedures allowed for accurate quantification of these drugs and identified important education points for future home-based sample collections.