4.6 Article

The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Predicting protein-membrane interfaces of peripheral membrane proteins using ensemble machine learning

Alexios Chatzigoulas et al.

Summary: Abnormal protein-membrane attachment plays a role in cellular pathway dysfunction and diseases. Modulating protein-membrane interactions through machine learning algorithms offers a promising therapeutic strategy for previously undruggable peripheral membrane proteins. This study presents an ensemble machine learning methodology for predicting membrane-penetrating amino acids, achieving high accuracy in identifying these amino acids and providing potential insights for designing drug-like molecules.

BRIEFINGS IN BIOINFORMATICS (2022)

Article Plant Sciences

Voltage-dependent anion channel proteins associate with dynamic Bamboo mosaic virus-induced complexes

Hsiang-Chi Lee et al.

Summary: This study reveals the involvement of mitochondrial voltage-dependent anion channel proteins in the dynamic Bamboo mosaic virus (BaMV)-induced complex, which supports BaMV accumulation in Nicotiana benthamiana. The study also demonstrates that interaction with BaMV triple gene block protein 1 (TGBp1) can induce aggregation of the NbVDAC proteins and promote BaMV replication. These findings highlight the importance of mitochondrial proteins in viral complex formation and virus infection.

PLANT PHYSIOLOGY (2022)

Article Multidisciplinary Sciences

Capping pores of alphavirus nsP1 gate membranous viral replication factories

Rhian Jones et al.

Summary: Positive-sense single-stranded RNA viruses, such as coronaviruses, flaviviruses and alphaviruses, replicate and transcribe within virus-induced membranous organelles in host cells. The remodeling of host-cell membranes for organelle formation allows concentration of metabolites and proteins for viral RNA synthesis, while preventing detection by the cellular innate immune system. The structure of non-structural protein 1 (nsP1) of the alphavirus chikungunya virus reveals the mechanism of RNA capping and membrane binding, providing insights into viral replication complex formation and potential antiviral strategies.

NATURE (2021)

Article Biochemical Research Methods

The trRosetta server for fast and accurate protein structure prediction

Zongyang Du et al.

Summary: The trRosetta server is a web-based platform for fast and accurate protein structure prediction using deep learning and Rosetta, which distinguishes itself from other similar servers in terms of rapid and accurate prediction of novel structures.

NATURE PROTOCOLS (2021)

Article Virology

Cryo-electron microscopy of nodavirus RNA replication organelles illuminates positive-strand RNA virus genome replication

Nuruddin Unchwaniwala et al.

Summary: The nodavirus flock house virus serves as a model for studying membrane-bound, positive-strand RNA virus genome replication complexes; the research shows that the replication vesicle is tightly packed with dsRNA and releases ssRNA replication products through a crown of viral RNA replication proteins. Further studies show similar RNA replication protein complexes in other (+)RNA viruses, suggesting related mechanisms and significant implications for viral function, evolution, and control.

CURRENT OPINION IN VIROLOGY (2021)

Article Microbiology

Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1

Kuo Zhang et al.

Summary: The study revealed the structure of CHIKV protein nsP1 and its crucial role in viral RNA replication and transcription. nsP1 forms a ring-like structure that facilitates communication between the viral replication complex and host cells. This research provides important insights into RNA capping and complex assembly during virus infection.

CELL HOST & MICROBE (2021)

Article Virology

Identification of a Proline-Kinked Amphipathic α-Helix Downstream from the Methyltransferase Domain of a Potexvirus Replicase and Its Role in Virus Replication and Perinuclear Complex Formation

Ken Komatsu et al.

Summary: In this study, it was found that an amphipathic alpha-helix structure downstream from the methyltransferase core domain of PlAMV replicase is crucial for membrane association and virus replication. The conserved proline-kinked helical sequence among potexviruses plays a key role in forming a perinuclear complex during virus infection. However, the formation of this complex by the replicase alone does not necessarily indicate successful virus replication.

JOURNAL OF VIROLOGY (2021)

Review Cell Biology

Ultrastructural Features of Membranous Replication Organelles Induced by Positive-Stranded RNA Viruses

Van Nguyen-Dinh et al.

Summary: Intracellular pathogens rely heavily on host cell organelles and metabolites for successful infection and replication. Positive-strand RNA viruses often induce alterations to the membrane system to shield their replication intermediates and accumulate necessary factors for efficient replication, while also preventing antiviral effectors from accessing. The specific structure and source of membranes induced by these viruses differ in plant and animal cells, but the principle remains consistent across species.

CELLS (2021)

Article Multidisciplinary Sciences

Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication

Nuruddin Unchwaniwala et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Multidisciplinary Sciences

A molecular pore spans the double membrane of the coronavirus replication organelle

Georg Wolff et al.

SCIENCE (2020)

Review Biochemistry & Molecular Biology

Double-Membrane Vesicles as Platforms for Viral Replication

Georg Wolff et al.

TRENDS IN MICROBIOLOGY (2020)

Review Biochemistry & Molecular Biology

Multi-level regulation of cellular recognition of viral dsRNA

Alys Peisley et al.

CELLULAR AND MOLECULAR LIFE SCIENCES (2013)

Review Cell Biology

Virus factories: biogenesis and structural design

Isabel Fernandez de Castro et al.

CELLULAR MICROBIOLOGY (2013)

Review Plant Sciences

Top 10 plant viruses in molecular plant pathology

Karen-Beth G. Scholthof et al.

MOLECULAR PLANT PATHOLOGY (2011)

Review Microbiology

Organelle-Like Membrane Compartmentalization of Positive-Strand RNA Virus Replication Factories

Johan A. den Boon et al.

ANNUAL REVIEW OF MICROBIOLOGY, VOL 64, 2010 (2010)

Review Microbiology

Cytoplasmic Viral Replication Complexes

Johan A. den Boon et al.

CELL HOST & MICROBE (2010)

Review Biochemistry & Molecular Biology

Varied Movement Strategies Employed by Triple Gene Block-Encoding Viruses

Jeanmarie Verchot-Lubicz et al.

MOLECULAR PLANT-MICROBE INTERACTIONS (2010)

Article Virology

Analysis of potato virus X replicase and TGBp3 subcellular locations

Devinka Bamunusinghe et al.

VIROLOGY (2009)

Review Microbiology

Modification of intracellular membrane structures for virus replication

Sven Miller et al.

NATURE REVIEWS MICROBIOLOGY (2008)

Review Biotechnology & Applied Microbiology

Molecular biology of potexviruses: recent advances

Jeanmarie Verchot-Lubicz et al.

JOURNAL OF GENERAL VIROLOGY (2007)

Article Biochemistry & Molecular Biology

Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing

Todd Blevins et al.

NUCLEIC ACIDS RESEARCH (2006)

Article Biochemistry & Molecular Biology

Protein structure prediction and analysis using the Robetta server

DE Kim et al.

NUCLEIC ACIDS RESEARCH (2004)

Article Biochemistry & Molecular Biology

Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase

J Schmidt-Mende et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2001)