Computational and Experimental Approaches to Study the RNA Secondary Structures of RNA Viruses

Most pandemics of recent decades can be traced to RNA viruses, including HIV, SARS, influenza, dengue, Zika, and SARS-CoV-2. These RNA viruses impose considerable social and economic burdens on our society, resulting in a high number of deaths and high treatment costs. As these RNA viruses utilize an RNA genome, which is important for different stages of the viral life cycle, including replication, translation, and packaging, studying how the genome folds is important to understand virus function. In this review, we summarize recent advances in computational and high-throughput RNA structure-mapping approaches and their use in understanding structures within RNA virus genomes. In particular, we focus on the genome structures of the dengue, Zika, and SARS-CoV-2 viruses due to recent significant outbreaks of these viruses around the world.

Automated summary

This review article summarizes the importance of RNA viruses and their impact on society and the economy. It presents the significance of studying genome folding and the use of computational and high-throughput RNA structure-mapping approaches. The focus is on the genome structures of dengue, Zika, and SARS-CoV-2 viruses.