4.5 Article

Insight towards the effect of the multi basic cleavage site of SARS-CoV-2 spike protein on cellular proteases

Journal

VIRUS RESEARCH
Volume 318, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.virusres.2022.198845

Keywords

Spike protein; Proteolytic activation; Furin; Cathepsin B; Plasmin; MD simulations

Categories

Funding

  1. Department of Biotechnology, India [BT/PR24308/NER/95/644/2017, BT/PR41246/NER/95/1685/2020]
  2. Department of Health Research, Government of India [NER/71/2020-ECD-I]

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This study analyzed the interaction between the S protein of SARS-CoV-2 and different host proteases using molecular docking and molecular dynamics simulation. It found that mutations in the sensitive cleavage site of the S protein can disrupt the binding of cellular proteases. This mutation study may help generate an attenuated SARS-CoV-2 and targeting host proteases with inhibitors could be a practical approach to stop the cellular spread of the virus and develop antiviral treatments.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection presents an immense global health problem. Spike (S) protein of coronavirus is the primary determinant of its entry into the host as it consists of both receptor binding and fusion domain. Besides tissue tropism, and host range, coronavirus pathogenesis are primarily controlled by the interaction of S protein with the cell receptor. Moreover, the proteolytic activation of S protein by host cell proteases plays a decisive role. The host-cell proteases have shown to be involved in the proteolysis of S protein and cleaving it into two functional subunits, S1 and S2, during the maturation process. In the present study, the interaction of the S protein of SARS-CoV-2 with different host proteases like furin, cathepsin B, and plasmin has been analyzed using molecular docking and molecular dynamics (MD) simulation. Incorporation of the furin cleavage site (R-R-A-R) in the S protein of SARS-CoV-2 has been studied by mutating the individual amino acid. MD simulation results suggest the polytropic nature of the S protein. Our analysis indicated that a single amino acid substitution in the polybasic cleavage site of S protein perturb the binding of cellular proteases. This mutation study might help to generate an attenuated SARS-CoV-2. Besides, targeting host proteases by inhibitors may result in a practical approach to stop the cellular spread of SARS-CoV-2 and develop its antiviral.

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