Fitness cost conferred by the novel erm(51) and rpoB mutation on environmental multidrug resistant-Rhodococcus equi

Rhodococcus equi is a common cause of severe pneumonia in foals. Emergence of macrolide-resistant R. equi isolated from foals and their environment has been reported in the United States. A novel erm(51) gene was recently identified in R. equi in soil from horse farms in Kentucky. Our objective was to determine the effect of the erm(51) gene and associated rpoB mutation on the fitness of multidrug resistant-R. equi (MDR-R. equr(.erm(51)+,) (rpoB+)) under different nutrient conditions. Bacterial growth curves were generated for 3 MDR-R. equi(.erm(51)+,) (rpoB+) isolates and 3 wild-type (WTN) R. equi isolates recovered from environmental samples of farms in central Kentucky. Growth was measured over 30.5 h in brain-heart infusion broth (BHI), minimal medium (MM), and minimal medium without iron (MM-I). All isolates had significantly (P < 0.05) higher growth in BHI compared to either MM or MM-I. MDR-R. equi(.erm(51)+,) (rpoB+) exhibited significantly lower growth compared to WTN isolates in BHI (nutrient-rich condition), but not in either MM or MM-I (nutrient-restricted conditions). This study indicates that under nutrient-rich conditions fitness of MDR-R. equi(.erm(51)+,) (rpoB+) is reduced relative to susceptible isolates; however, under nutrient-restricted conditions MDR-R. equi(.erm(51)+,) (rpoB+) isolates grow similarly to susceptible isolates. These findings indicate that MDR-R. equi(.erm(51)+,) (rpoB+) might be outcompeted by susceptible isolates in nature when practices to reduce antimicrobial pressure, such as reducing antimicrobial use in foals, are implemented. But it also raises the concern that these resistant genotypes might persist in the environment of horse-breeding farms in the face of selective pressures such as antimicrobials or nutrient restriction.

Automated summary

The study found that under nutrient-rich conditions, fitness of MDR-R. equi(.erm(51)+,) (rpoB+) is reduced relative to susceptible isolates, but under nutrient-restricted conditions, they grow similarly. This suggests that resistant isolates might be outcompeted by susceptible ones in nature when antimicrobial use is reduced, but could persist in farm environments.