GPR35, ally of the anti-ischemic ATPIF1-ATP synthase interaction

Review Cell Biology

Molecular mechanisms and consequences of mitochondrial permeability transition

Massimo Bonora et al.

Summary: Mitochondrial permeability transition, mediated by the opening of the mitochondrial permeability transition pore, causes flux of low molecular weight solutes across the inner mitochondrial membrane, triggering various cellular responses. Recent studies have shed light on the molecular nature and mechanisms of the mitochondrial permeability transition pore, paving the way for understanding mitochondrial biology and its pathophysiological consequences.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2022)

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Article Multidisciplinary Sciences

Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists

Gregory A. Wyant et al.

Summary: Kynurenic acid (KynA) has tissue-protective effects in ischemia models, and this study demonstrates that activation of GPR35 and interaction with ATPIF1 may be the underlying mechanism. These findings provide a rationale for developing specific GPR35 agonists for the treatment of ischemic diseases.

SCIENCE (2022)

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Article Biochemistry & Molecular Biology

Mitochondrial metabolism and bioenergetic function in an anoxic isolated adult mouse cardiomyocyte model of in vivo cardiac ischemia-reperfusion injury

Anja V. Gruszczyk et al.

Summary: Cell models of cardiac ischemia-reperfusion injury are crucial for studying cardiac pathology, however, current monolayer cell models are inadequate in replicating in vivo injury. This study identified two reasons for the limitations: the presence of oxygen in cellular hypoxia models sustains mitochondrial oxidative phosphorylation, and the loss of lactate from cells during ischemia sustains glycolysis. By incubating isolated adult mouse cardiomyocytes anoxically and inhibiting lactate efflux, key markers of in vivo ischemia were recapitulated, allowing for the assessment of important aspects of cardiac IR injury in vitro.

REDOX BIOLOGY (2022)

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Article Biochemistry & Molecular Biology

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome

Edward L. Huttlin et al.

Summary: This study explores thousands of interactions between proteins, creating two essential cell-line specific interaction networks that help researchers understand the principles of proteome organization and unknown protein characteristics. Comparing different cell lines revealed extensive customization, linking core complexes with essential proteins and rewiring subnetworks to produce each cell's phenotype.

CELL (2021)

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Article Biochemistry & Molecular Biology