Journal
THERIOGENOLOGY
Volume 195, Issue -, Pages 199-208Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2022.10.030
Keywords
ASSES GENETIC Preservation; Metabolomics; SPERM INJECTION; EQUUS WILD Conservation; Transvaginal OOCYTE ASPIRATION; IN VITRO EMBRYO PRODUCTION
Categories
Ask authors/readers for more resources
This study investigated the effect of supplementation with jenny preovulatory follicular fluid (PFF) on oocyte developmental competence in donkeys. The results showed that IVM media supplemented with FBS or donkey PFF can promote nuclear maturation and early embryo development in donkeys. This study is the first report of ICSI, time-lapse imaging, and in vitro blastocyst production in donkeys.
Equus members exhibit very divergent karyotype, genetic plasticity, and significant differences in their reproductive physiology. Despite the fact that somatic cell nuclear transfer and intracytoplasmic sperm injection (ICSI) has gained relevance in the last few years in horses, few reports have been published exploring ovum pick up (OPU) and in vitro maturation (IVM) of cumulus-oocyte complexes (COCs) in donkeys. Yet, some donkey species and breeds are considered endangered, and these assisted-reproductive technologies could help to preserve the genetic of valuable individuals. In this study, we tested the hypothesis that supplementation with jenny preovulatory follicular fluid (PFF) during IVM could improve oocyte developmental competence in the donkey. For this, in vitro nuclear maturation rates, cumulus cell expansion, and embryo development after ICSI of donkey COCs matured in culture media supplemented with fetal bovine serum (FBS) or donkey PFF, with a known metabolomic profile, were assessed. Time-lapse imagining was performed after ICSI of horse and donkey oocytes. Eight OPU sessions were done in five jennies with an average recovery rate of 69.2% (n = 45 COCs). Although lower cumulus cells expansion was observed in oocytes of PFF group (P = 0.0010), no significant differences were described in nuclear maturation rates and preimplantation embryo development between groups. Donkey ICSI embryos showed similar morphokinetics to horse ICSI embryos. Our study shows that supplementing IVM media with FBS or donkey PFF supports nuclear maturation and early preimplan-tation embryo development after ICSI in donkeys. To our knowledge, the present study is the first report of ICSI, time-lapse imaging and in vitro blastocyst production in donkey.(c) 2022 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available