Disruption of Leydig cell steroidogenic function by sodium arsenite and/or sodium fluoride

Arsenite (As) and fluoride (F), both of which are linked to a variety of human ailments, are regularly found in underground drinking water. Numerous studies have shown that As and/or F have negative impacts on testicular function and fertility. For this purpose, mouse Leydig cells, the main cells responsible for the generation and regulation of steroid hormones such as testosterone, were used to reveal the effects of individual and combined exposure of As and F on the steroidogenic pathway in the male reproductive system. Leydig cells were treated with 0.39 mM (50 ppb) As and 0.0476 mM (2 ppm) F alone and in combination for 24 h. The findings revealed that As and/or F exposure induced oxidative stress and apoptosis in Leydig cells and altered antioxidant equilibrium of the cells by reducing super-oxide dismutase, catalase, glutathione peroxidase. Additionally, individual and combined administration of As and/or F significantly supressed the expression of both steroidogenic enzymes and the genes encoding these enzymes. In conclusion, this study showed that exposure to As and F at environmentally relevant concentrations dispersed by water decreased testosterone production in Leydig cells, an important cell of the male reproductive system. The deleterious effects of even the lowest concentrations of As and F elements that can reach humans from the environment on the Leydig cell, and therefore on male infertility, emphasize necessity new safe limits for these elements.(c) 2022 Elsevier Inc. All rights reserved.

Automated summary

Arsenite and fluoride, commonly found in underground drinking water, have negative impacts on testicular function and fertility. In mouse Leydig cells, exposure to individual or combined arsenite and fluoride induces oxidative stress, cell apoptosis, alters antioxidant balance, and suppresses steroidogenic enzyme expression. This study highlights the detrimental effects of environmentally relevant concentrations of arsenite and fluoride on testosterone production in Leydig cells and male infertility.